Enhanced chemiluminescence: a high-sensitivity detection system for in situ hybridization and immunohistochemistry.

摘要: The breakthrough of chemiluminescence in the field solution immunoassays and transfer membranes prompted us to explore whether a light-based detection system could provide gain sensitivity over chromogenic FITC markers for nucleic acid protein on histological preparations. A Hamamatsu device an enhanced (ECL) luminol substrate peroxidase were used detect epithelial endothelial components by immunohistochemistry (IHC) situ hybridization (ISH) papilloma virus DNA. accuracy signal was compared that obtained with DAB-peroxidase, silver-enhanced NBT-BCIP-alkaline phosphatase, FITC. Our results demonstrated feasibility high luminescence In part due ultrasensitive videocamera photon-counting imaging, interpretable reproducible within counting times shorter than 5 min, dilutions primary antibodies 100- 10,000-fold greater those reactions. As ISH, identical concentrations HPV 18 DNA probe HeLa cells, labeling apparent but undetectable chromogen. morphological resolution allowed discriminatory analysis signal. Therefore, at light microscopic level, offers appealing alternative quantification low-concentration molecules.