Directed Evolution and Mutant Characterization of Nitrilase from Rhodococcus rhodochrous tg1-A6
作者： Hui Luo , Jinwei Ma , Yanhong Chang , Huimin Yu , Zhongyao Shen
DOI: 10.1007/S12010-015-1964-9
关键词: Biochemistry 、 Mutant 、 Rhodococcus rhodochrous 、 Rhodococcus 、 Glycolonitrile 、 Directed evolution 、 Wild type 、 Biology 、 Nitrilase 、 Amino acid
摘要: … coli BL21(DE3)/pET-Nit containing the wild-type nitrilase gene from R. rhodochrous tg1-A6 … The plasmid pET-Nit with the wild-type nitrilase gene from R. rhodochrous tg1-A6 was …
springer.com
sci-hub.se 参考文章(26)
Frances H. Arnold, George Georgiou, Directed enzyme evolution : screening and selection methods Humana Press. ,(2003)
Andrew D Griffiths, Samuel C Williams, Oliver Hartley, Ian M Tomlinson, Peter Waterhouse, William L Crosby, Roland E Kontermann, Peter T Jones, Nigel M Low, T John Allison, Terence D Prospero, Hennie R Hoogenboom, Ahuva Nissim, Jonathan PL Cox, Jacqueline L Harrison, Manuela Zaccolo, Ermanno Gherardi, Greg Winter, Isolation of high affinity human antibodies directly from large synthetic repertoires. The EMBO Journal. ,vol. 13, pp. 3245- 3260 ,(1994) , 10.1002/J.1460-2075.1994.TB06626.X
Ye Li, Patrick C. Cirino, Recent advances in engineering proteins for biocatalysis Biotechnology and Bioengineering. ,vol. 111, pp. 1273- 1287 ,(2014) , 10.1002/BIT.25240
Nitya Nand Sharma, Monica Sharma, Tek Chand Bhalla, An improved nitrilase-mediated bioprocess for synthesis of nicotinic acid from 3-cyanopyridine with hyperinduced Nocardia globerula NHB-2 Journal of Industrial Microbiology & Biotechnology. ,vol. 38, pp. 1235- 1243 ,(2011) , 10.1007/S10295-010-0902-7
Bruno C. M. Fernandes, Cesar Mateo, Christoph Kiziak, Andrzej Chmura, Jan Wacker, Fred van Rantwijk, Andreas Stolz, Roger A. Sheldon, Nitrile Hydratase Activity of a Recombinant Nitrilase Advanced Synthesis & Catalysis. ,vol. 348, pp. 2597- 2603 ,(2006) , 10.1002/ADSC.200600269
Hui Luo, Lu Fan, Yanhong Chang, Jinwei Ma, Huimin Yu, Zhongyao Shen, Gene Cloning, Overexpression, and Characterization of the Nitrilase from Rhodococcus rhodochrous tg1-A6 in E. coli Applied Biochemistry and Biotechnology. ,vol. 160, pp. 393- 400 ,(2010) , 10.1007/S12010-008-8324-Y
Zhi-Jun Zhang, Jian-He Xu, Yu-Cai He, Li-Ming Ouyang, You-Yan Liu, Cloning and biochemical properties of a highly thermostable and enantioselective nitrilase from Alcaligenes sp. ECU0401 and its potential for (R)-(−)-mandelic acid production Bioprocess and Biosystems Engineering. ,vol. 34, pp. 315- 322 ,(2011) , 10.1007/S00449-010-0473-Z
Huihui Sun, Wenyuan Gao, Haiyang Fan, Hualei Wang, Dongzhi Wei, Cloning, purification and evaluation of the enzymatic properties of a novel arylacetonitrilase from Luminiphilus syltensis NOR5-1B: a potential biocatalyst for the synthesis of mandelic acid and its derivatives Biotechnology Letters. ,vol. 37, pp. 1655- 1661 ,(2015) , 10.1007/S10529-015-1830-4
H. Komeda, Y. Hori, M. Kobayashi, S. Shimizu, Transcriptional regulation of the Rhodococcus rhodochrous J1 nitA gene encoding a nitrilase Proceedings of the National Academy of Sciences of the United States of America. ,vol. 93, pp. 10572- 10577 ,(1996) , 10.1073/PNAS.93.20.10572
Ana Fernandez-Gacio, Marilyne Uguen, Jacques Fastrez, Phage display as a tool for the directed evolution of enzymes Trends in Biotechnology. ,vol. 21, pp. 408- 414 ,(2003) , 10.1016/S0167-7799(03)00194-X