An assay for determination of rat adrenal catechol-O-methyltransferase activity: Comparison of spontaneously hypertensive rats and Wistar-Kyoto rats
作者： Makoto Tsunoda , Kazuhiro Imai
DOI: 10.1007/S00216-004-2884-7
关键词: Enzyme 、 Catechol-O-methyl transferase 、 Epinephrine 、 Adrenal gland 、 Methyltransferase 、 Internal medicine 、 Metanephrine 、 High-performance liquid chromatography 、 Chromatography 、 Wistar Kyoto Rats 、 Chemistry 、 Endocrinology
摘要: A method has been developed for measurement of catechol-O-methyltransferase (COMT) activity in the rat adrenal gland. Epinephrine, synthesized gland, was used as substrate, and its enzymatic product, metanephrine, quantified by high-performance liquid chromatography (HPLC) with fluorescence detection. The sufficient precision accuracy. Soluble (S) membrane-bound (MB) COMT Wistar–Kyoto (WKY) rats 20.7±3.5 18.6±3.4 pmol min−1 mg−1 protein (n=5), respectively. To clarify role blood-pressure regulation, S MB spontaneously hypertensive were determined. Respective 17.0±1.1 which is similar to that WKY rats. This finding suggests gland might not be related blood pressure regulation.
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