Methods for phrasing epigenetic modifications of genomes

作者: Wei Zhou , Justin Costa , Jeremy Edwards

DOI:

关键词: BiologySequence (medicine)Computational biologyEpigeneticsGenomeAffinity bindingBarcodeNucleic acidEpigenome

摘要: Provided herein are methods and compositions for analyzing epigenetic modifications of genomes. The suited complete epigenome sequencing any modification which an antibody or affinity binding agent has been developed. In one aspect, provided is a method genome. some embodiments aspects herein, the further comprises library to generate sequence reads, assembling reads with aid positional barcode information. determining location at least two different nucleic acid. Another aspect present disclosure provides kit

参考文章(21)
Ian Kavanagh, Heli T. Haakana, Laura-Leena Kiiskinen, Methods and transposon nucleic acids for generating a dna library ,(2012)
Stephen Y. Chou, Zhaoning Yu, Han Cao, Jonas O. Tegenfeldt, Robert H. Austin, Nanochannel arrays and their preparation and use for high throughput macromolecular analysis ,(2002)
Kalim Mir, Arrays and methods of use ,(2002)
Benjamin A Flusberg, Dale R Webster, Jessica H Lee, Kevin J Travers, Eric C Olivares, Tyson A Clark, Jonas Korlach, Stephen W Turner, Direct detection of DNA methylation during single-molecule, real-time sequencing Nature Methods. ,vol. 7, pp. 461- 465 ,(2010) , 10.1038/NMETH.1459
Gang Fang, Diana Munera, David I Friedman, Anjali Mandlik, Michael C Chao, Onureena Banerjee, Zhixing Feng, Bojan Losic, Milind C Mahajan, Omar J Jabado, Gintaras Deikus, Tyson A Clark, Khai Luong, Iain A Murray, Brigid M Davis, Alona Keren-Paz, Andrew Chess, Richard J Roberts, Jonas Korlach, Steve W Turner, Vipin Kumar, Matthew K Waldor, Eric E Schadt, Genome-wide mapping of methylated adenine residues in pathogenic Escherichia coli using single-molecule real-time sequencing Nature Biotechnology. ,vol. 30, pp. 1232- 1239 ,(2012) , 10.1038/NBT.2432
Shuang Fang Lim, Alena Karpusenko, John J. Sakon, Joseph A. Hook, Tyra A. Lamar, Robert Riehn, DNA methylation profiling in nanochannels Biomicrofluidics. ,vol. 5, pp. 034106- ,(2011) , 10.1063/1.3613671
Danielle Queiroz Calcagno, Carolina Oliveira Gigek, Elizabeth Suchi Chen, Rommel Rodriguez Burbano, Marília de Arruda Cardoso Smith, DNA and histone methylation in gastric carcinogenesis World Journal of Gastroenterology. ,vol. 19, pp. 1182- 1192 ,(2013) , 10.3748/WJG.V19.I8.1182
Gene E Ananiev, Steve Goldstein, Rod Runnheim, Dan K Forrest, Shiguo Zhou, Konstantinos Potamousis, Chris P Churas, Veit Bergendahl, James A Thomson, David C Schwartz, Optical mapping discerns genome wide DNA methylation profiles BMC Molecular Biology. ,vol. 9, pp. 68- 68 ,(2008) , 10.1186/1471-2199-9-68