Immobilization of Enzymes, Microbial Cells, and Organelles by Inclusion with Photo-Crosslinkable Resins
作者： Saburo Fukui , Atsuo Tanaka , Gerald Gellf
DOI: 10.1007/978-1-4684-6985-1_61
关键词: Suspension (chemistry) 、 Macromolecule 、 Isophorone diisocyanate 、 Organelle 、 Alcohol oxidase 、 Enzyme 、 Chemistry 、 Combinatorial chemistry
摘要: This communication deals with the application of photo-crosslinkable resins to inclusion enzymes, microbial cells and organelles. The method consists mixing (a) liquid oligomers suitable resin(s) containing photo-sensitive functional groups, (b) an appropriate initiator, (c) enzyme solution or suspension organelles, followed by illumination near-ultraviolet light for only a few minutes. simple convenient procedure produces tailor-made matrices in which biologically active macromolecules are successfully entrapped.
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