Type I IFN-Producing CD4 Vα14i NKT Cells Facilitate Priming of IL-10-Producing CD8 T Cells by Hepatocytes

摘要: Upon entering the liver CD8 T cells encounter large numbers of NKT patrolling hepatocyte (HC) surface facing perisinusoidal space. We asked whether hepatic modulate priming by HC. Hepatic (α-galactosyl-ceramide-loaded CD1d dimer binding) produce predominantly IL-4 when stimulated with glycolipid-presenting HC but IFN-γ dendritic cells. These prime naive to a (Kb-presented) peptide ligand if they simultaneously recognize CD1d-binding glycolipid presented them on responding that prime. No IL-10-producing are detected these primed either or In contrast, IL-10 is produced HC-primed IFN-β-producing coactivated same presenting (in context CD1d) and an antigenic Kb). Hence, generated in type I IFN-dependent manner three cell types (CD8 cells, ligand-presenting HC) specifically closely interact. under conditions down-modulate IL-2 (and proliferative) responses CD4 DC. If close proximity, can thus locally phenotype during their thereby limiting local activation proinflammatory immune effector protecting against injury.