A unique population of bone marrow cells migrates to skeletal muscle via hepatocyte growth factor/c-met axis.

摘要: Cells expressing the CD45-associated hematopoietic marker are predominantly present in mammalian bone marrow (BM), but have recently been shown to also reside skeletal muscle and potentially participate repair. Despite consistent observations, specific relationship potential migration of CD45+ cells BM versus residing remain unclear, addition any understanding factors that may regulate trafficking CD45+-derived upon i.v. transplantation. Here, transplantation BM-derived fully replaced fraction muscle, gave rise progenitor with distinct lineage capacity from BM. Using transwell assays, a subset was migrate exclusively mature not stromal cells. Unlike stroma, myofiber induced affected by stromal-derived factor-1 (SDF-1) neutralization or CXCR4-blocking antibody, could be reduced c-met-blocking antibody augmented hepatocyte growth factor (HGF), putative ligand for c-met. We suggest compartment consists functionally complex population progenitors includes HGF/c-met responsive capable muscle. This previously unappreciated basis cellular tracking now aids defining regulatory networks distinguish stem cell niche microenvironments.