Astrocyte-regulated synaptogenesis: an in vitro ultrastructural study
作者： M. Rouget , D. Araud , R. Seite , A. Prochiantz , A. Autillo-Touati
DOI: 10.1016/0304-3940(93)90114-Z
关键词: Central nervous system 、 Neuroscience 、 Striatum 、 Neuron 、 Synaptogenesis 、 Astrocyte 、 Neuroglia 、 Cell culture 、 Biology 、 Chemically defined medium
摘要: Striatal neurons from E15 rat embryos were dissociated, plated at low cell density on polyornithine or astrocyte monolayers derived the striatum (homotopic) mesencephalon (heterotopic), and cultured in a chemically defined medium. Dendrites developing homotopic co-cultures could reach state of maturation allowing establishment synapses with axons mesencephalic explants. This culture system thus partially reproduces vivo conditions which striatal an glial environment can serve as synaptic targets for afferent axons.
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