New monolithic enzymatic micro-reactor for the fast production and purification of oligogalacturonides.
作者： C. Delattre , P. Michaud , M.A. Vijayalakshmi
DOI: 10.1016/J.JCHROMB.2007.08.036
关键词: Microreactor 、 Bioreactor 、 Chemistry 、 Context (language use) 、 Pectin lyase 、 Chromatography 、 Monolithic HPLC column 、 Oligosaccharide 、 Monolith 、 Uronic acid
摘要: Fast production and purification of alpha-(1,4)-oligogalacturonides was investigated using a new enzymatic reactor composed monolithic matrix. Pectin lyase from Aspergillus japonicus (Sigma) immobilized on CIM-disk epoxy monolith. Studies were performed free pectin to compare the optimum temperature, pH, thermal stability. It determined that temperature for support is 30 degrees C, pH 5. Monolithic chromatography one fastest liquid chromatographic method used separation biomolecules due high mass transfer rate. In this context, online step oligogalacturonides associating DEAE. This efficient bioreactor uronic oligosaccharides polygalacturonic acid (PGA) constitutes an original fast process generate bioactive oligouronides.
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