SOCS36E, a novel Drosophila SOCS protein, suppresses JAK/STAT and EGF-R signalling in the imaginal wing disc

摘要: We have cloned a novel SOCS gene from Drosophila, socs36E, which is most homologous to the mammalian socs-5 gene. Socs36E expressed zygotically, predominantly during embryogenesis, in highly dynamic pattern. In vivo expression of SOCS36E transgenic flies results several adult phenotypes. Engrailed-GAL4 directed causes loss wing anterior cross vein, humeral outgrowths, absence halteres and eye pigmentation defects. Expression under apterous-GAL4 control resulted outstretched wings. Full penetrance these phenotypes required presence SH2 SOCS-box domains SOCS36E. The observed were consistent with defects JAK/STAT or EGF-R signalling exacerbated heterozygous for either d-jak (hopscotch), d-stat (stat92E) d-egf-r (der) genes. Conversely, inactivating one copy d-cbl gene, negative regulator d-EGF-R, partially rescued These genetic interactions imply that can suppress activities pathways disc suggest interacts multiple vivo.