TNFα siRNA Reduces Brain TNF and EEG Delta Wave Activity in Rats

摘要: Abstract Tumor necrosis factor alpha (TNFα) is a pleiotropic cytokine with several CNS physiological and pathophysiological actions including sleep, memory, thermal appetite regulation. Short interfering RNAs (siRNA) targeting TNFα were incubated cortical cell cultures microinjected into the primary somatosensory cortex (SSctx) of rats. The siRNA treatment specifically reduced mRNA by 45% in vitro without affecting interleukin-6 or gluR1–4 levels. In vivo siRNAα mRNA, gluR1 levels compared to scrambled control siRNA. After microinjection, density TNFα-immunoreactive cells layer V SSctx was also reduced. Electroencephalogram (EEG) delta wave power decreased on days 2 3 side brain that received microinjection relative receiving These findings support hypothesis attenuates protein rat those reductions reduce EEG power. Results are consistent notion involved physiology sleep