Extract of Ganoderma lucidum prolongs sleep time in rats.

摘要: Abstract Ethnopharmacological relevance Ganoderma lucidum (Ling Zhi) is a basidiomycete white-rot macrofungus that has been used as tranquilizing agent (i.e., An-Shen effect) for the treatment of restlessness, insomnia, and palpitation in China hundreds years. Aim study The present aimed to investigate whether extract (GLE) influences sleep freely moving rats potential mechanism. Materials methods was extracted from fruiting bodies lucidum. Rats were treated with GLE orally 3 days, on third day, electroencephalographic electromyographic recordings made 6 h 9:00 p.m. 3:00 a.m. rats. Sleep parameters analyzed using SleepSign software. Tumor necrosis factor-α (TNF-α) levels measured enzyme-linked immunosorbent assay. Results Three-day administration significantly increased total time non-rapid eye movement (NREM) at dose 80 mg/kg (i.g.) without influencing slow-wave or REM TNF-α concomitantly serum, hypothalamus, dorsal raphe nucleus. hypnotic effect (80 mg/kg, i.g.) inhibited by intracerebroventricular injection antibody (2.5 μg/rat). Co-administration (40 mg/kg, (12.5 ng/rat, i.c.v.), both ineffective doses, revealed an additive effect. Conclusion These results suggest effects mechanism which promoted remains unclear, but this appears be primarily related modulation cytokines such TNF-α. Furthermore, these data least partially support ethnomedical use