Determination of Lipid Hydroperoxides in Marine Diatoms by the FOX2 Assay.
作者： Ida Orefice , Andrea Gerecht , Giuliana d'Ippolito , Angelo Fontana , Adrianna Ianora
DOI: 10.3390/MD13095767
关键词: Skeletonema marinoi 、 Lipoxygenase 、 Biochemistry 、 Beta oxidation 、 Diatom 、 Copepod 、 Biology 、 Fatty acid 、 Phenols 、 Ferrous Compounds
摘要: Ecologically-relevant marine diatoms produce a plethora of bioactive oxylipins deriving from fatty acid oxidation, including aldehydes, hydroxy-fatty acids, epoxy-hydroxy-fatty and oxo-acids. These secondary metabolites have been related to the negative effect on copepod reproduction, causing low hatching success teratogenesis in offspring during periods intense diatom blooms. The common intermediates formation are hydroperoxides. quantitative measurement these can fundamentally contribute understanding function role lipoxygenase diatom-copepod interactions. Here, we describe successful adaptation ferrous oxidation-xylenol orange 2 (FOX2) assay samples, which showed several advantages over other spectrophotometric polarographic methods tested present work. Using this method assessed hydroperoxide levels three species: Skeletonema marinoi, Thalassiosira rotula, Chaetoceros affinis, discuss results light literature data their detrimental effects reproduction.
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