Specificity of Lipoxygenase Pathways Supports Species Delineation in the Marine Diatom Genus Pseudo-nitzschia

摘要: Oxylipins are low-molecular weight secondary metabolites derived from the incorporation of oxygen into carbon chains polyunsaturated fatty acids (PUFAs). produced in many prokaryotic and eukaryotic lineages where they involved a broad spectrum actions spanning stress defense responses, regulation growth development, signaling, innate immunity. We explored diversity oxylipin patterns marine planktonic diatom Pseudo-nitzschia. This genus includes several species only distinguishable with aid molecular markers. Oxylipin profiles cultured strains were obtained by reverse phase column on liquid chromatograph equipped UV photodiode detector q-ToF mass spectrometer. Lipoxygenase compounds mapped phylogenies Pseudo-nitzschia inferred nuclear encoded hyper-variable region LSU rDNA plastid rbcL. Results showed that exhibits rich varied lipoxygenase metabolism eicosapentaenoic acid (EPA), high level specificity for markers generally corroborated genotypic delineation, even among genetically closely related cryptic species. These results suggest constitute additional identification tools providing functional support to delineation morphological traits. The exploration diversity, plasticity production across genera will also provide insights ecological functions these selective pressures driving their diversification.