The Influence of Hyaluronic Acid and Glioblastoma Cell Coculture on the Formation of Endothelial Cell Networks in Gelatin Hydrogels.
作者： Mai T. Ngo , Brendan A. Harley
关键词: In vivo 、 Self-healing hydrogels 、 Hyaluronic acid 、 Materials science 、 Umbilical vein 、 Angiogenesis 、 Vasculogenesis 、 Immunology 、 Endothelial stem cell 、 Cell biology 、 Vascular endothelial growth factor
摘要: Glioblastoma (GBM) is the most common and deadly form of brain cancer. Interactions between GBM cells vasculature in vivo contribute to poor clinical outcomes, with GBM-induced vessel co-option, regression, subsequent angiogenesis strongly influencing invasion. Here, elements perivascular niche are incorporated into a methacrylamide-functionalized gelatin hydrogel as means examine GBM-vessel interactions. The complexity 3D endothelial cell networks formed from human umbilical vein normal lung fibroblasts function properties vascular growth factor (VEGF) presentation presented. While overall length branching decrease increasing stiffness incorporation brain-mimetic hyaluronic acid, it can be separately altered by changing seeding density. It shown that covalent VEGF supports network formation robustly continuously available soluble VEGF. impact U87-MG on subsequently investigated. localize proximity hasten regression vitro. Together, this vitro platform recapitulates close association structures well co-option preceding vivo.
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