Conservation and diversification of Wnt signaling function during the evolution of nematode vulva development.

摘要: Cell-fate specification and cell-cell signaling have been well studied during vulva development in Caenorhabditis elegans provide a paradigm evolutionary developmental biology1,2. Pristionchus pacificus has developed as 'satellite' organism with an integrated physical genetic map that allows detailed comparisons to C. elegans3,4,5. A common aspect of formation both species is the polarization P7.p lineage, which responsible for vulval symmetry. In elegans, Wnt crucial cell-fate patterning6; nothing known about symmetry P. pacificus. We isolated mutations disrupt lineage found corresponding gene encodes Frizzled-like molecule. addition, Ppa-lin-17 (encoding Frizzled) morpholino knock-down Ppa-lin-44 Wnt), Ppa-egl-20 Ppa-mig-5 Dsh), Ppa-apr-1 APC) Ppa-bar-1 β-catenin) results gonad-independent differentiation, indicating these genes role negative process. contrast, positive induction, bar-1 result hypoinduced phenotype7. Therefore, whereas molecular mechanisms generate are conserved, control induction diversified evolution.