Pseudomonas aeruginosa Alters Its Transcriptome Related to Carbon Metabolism and Virulence as a Possible Survival Strategy in Blood from Trauma Patients

摘要: ABSTRACT Trauma patients (TPs) are highly susceptible to infections, which often lead sepsis. Among the numerous causative agents, Pseudomonas aeruginosa is especially important, as P. sepsis fatal. Understanding mechanism of its pathogenesis in bloodstream infections imperative; however, this has not been previously described. To examine effect trauma-induced changes blood on expression genes, we grew strain UCBPP-PA14 (PA14) samples from eight TPs and seven healthy volunteers (HVs). Compared with growth HVs, PA14 significantly altered 285 genes. Genes whose was increased were related carbon metabolism, malonate utilization mannitol uptake, efflux heavy metals. reduced included genes type VI secretion system, uptake metabolism amino acids, biosynthesis transport siderophores pyoverdine pyochelin. These results suggest that during systemic infection trauma patients, adapt blood, adjusts positively negatively virulence, respectively. IMPORTANCE While a considerable body knowledge regarding available, potential influence these basically an unexplored area. Rather than using standard laboratory media, whole either or patients. The specific transcriptome response reflect adaptation organism environment. This vital for understanding strategies pathogen uses survive within host infection. Such information will help researchers clinicians develop new approaches treatment caused by terms recognizing effects therapies (e.g., iron, zinc, mannitol) organism. Further, can most likely be extrapolated all septicemia. Author Video: An author video summary article available.