The C/D box small nucleolar RNA SNORD52 regulated by Upf1 facilitates Hepatocarcinogenesis by stabilizing CDK1.
作者： Cuicui Li , Long Wu , Pengpeng Liu , Kun Li , Zhonglin Zhang
DOI: 10.7150/THNO.47677
关键词: Cyclin-dependent kinase 1 、 RNA 、 Downregulation and upregulation 、 Cancer research 、 KEGG 、 Carcinogenesis 、 Small nucleolar RNA 、 Cell Cycle Gene 、 Biology 、 Cell culture
摘要: Rationale: Understanding the roles of small nucleolar RNAs (snoRNAs) in hepatocarcinogenesis will provide new avenues to identify diagnostic and therapeutic targets for hepatocellular carcinoma (HCC). Our previous research confirmed tumor-suppressive effect Up-frameshift 1 (Upf1) HCC. Herein, we examined expression profiles snoRNAs regulated by Upf1 hepatoma cells. Methods: We cells using RNA-sequencing analysis then investigated significance SNORD52 HCC tissue different cell lines. The protumorigenic effects on were both vitro vivo gain-of-function loss-of-function assays. RNA pull-down assays mass spectrometry used RNA-binding protein that binds SNORD52. Results: Many identified; one which, human C/D box SNORD52, was upregulated tissues negatively correlated with expression, patients higher had a poor clinical prognosis. promoted tumorigenesis vivo. Mechanistically, KEGG showed series cycle genes further CDK1 enhancing stability proteins function depends presence CDK1. Conclusion: Overall, present study indicates could be potential biomarker Targeting Upf1/SNORD52/CDK1 pathway might have
thno.org参考文章(44)
Di Zheng, Jie Zhang, Jian Ni, Jie Luo, Jiying Wang, Liang Tang, Ling Zhang, Li Wang, Jianfang Xu, Bo Su, Gang Chen, Small nucleolar RNA 78 promotes the tumorigenesis in non-small cell lung cancer Journal of Experimental & Clinical Cancer Research. ,vol. 34, pp. 49- 49 ,(2015) , 10.1186/S13046-015-0170-5
Giorgio Dieci, Milena Preti, Barbara Montanini, Eukaryotic snoRNAs: a paradigm for gene expression flexibility. Genomics. ,vol. 94, pp. 83- 88 ,(2009) , 10.1016/J.YGENO.2009.05.002
Tomaž Bratkovič, Boris Rogelj, The many faces of small nucleolar RNAs Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms. ,vol. 1839, pp. 438- 443 ,(2014) , 10.1016/J.BBAGRM.2014.04.009
Jean-Pierre Bachellerie, Monique Nicoloso, Liang-Hu Qu, Bernard Michot, Michèle Caizergues-Ferrer, Jérome Cavaille, Marie-Hélène Renalier, Novel intron-encoded small nucleolar RNAs with long sequence complementarities to mature rRNAs involved in ribosome biogenesis. Biochemistry and Cell Biology. ,vol. 73, pp. 835- 843 ,(1995) , 10.1139/O95-091
Christoph Schweingruber, Simone C. Rufener, David Zünd, Akio Yamashita, Oliver Mühlemann, Nonsense-mediated mRNA decay — Mechanisms of substrate mRNA recognition and degradation in mammalian cells Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms. ,vol. 1829, pp. 612- 623 ,(2013) , 10.1016/J.BBAGRM.2013.02.005
Xue-Yuan Dong, Peng Guo, Jeff Boyd, Xiaodong Sun, Qunna Li, Wei Zhou, Jin-Tang Dong, Implication of snoRNA U50 in human breast cancer Journal of Genetics and Genomics. ,vol. 36, pp. 447- 454 ,(2009) , 10.1016/S1673-8527(08)60134-4
Lara B Weinstein, Joan A Steitz, Guided tours: from precursor snoRNA to functional snoRNP. Current Opinion in Cell Biology. ,vol. 11, pp. 378- 384 ,(1999) , 10.1016/S0955-0674(99)80053-2
Lijun Wang, Yu Zhao, Xichen Bao, Xihua Zhu, Yvonne Ka-yin Kwok, Kun Sun, Xiaona Chen, Yongheng Huang, Ralf Jauch, Miguel A Esteban, Hao Sun, Huating Wang, LncRNA Dum interacts with Dnmts to regulate Dppa2 expression during myogenic differentiation and muscle regeneration Cell Research. ,vol. 25, pp. 335- 350 ,(2015) , 10.1038/CR.2015.21
Akio Yamashita, Role of SMG‐1‐mediated Upf1 phosphorylation in mammalian nonsense‐mediated mRNA decay Genes to Cells. ,vol. 18, pp. 161- 175 ,(2013) , 10.1111/GTC.12033
Søren Lykke-Andersen, Yun Chen, Britt R. Ardal, Berit Lilje, Johannes Waage, Albin Sandelin, Torben Heick Jensen, Human nonsense-mediated RNA decay initiates widely by endonucleolysis and targets snoRNA host genes Genes & Development. ,vol. 28, pp. 2498- 2517 ,(2014) , 10.1101/GAD.246538.114