MUC4 and MUC1 expression in adenocarcinoma of the stomach correlates with vessel invasion and lymph node metastasis: an immunohistochemical study of early gastric cancer.

摘要: We have previously reported that MUC4 expression is a poor prognostic factor in various carcinomas. Our previous study also showed MUC1 gastric cancers, including the early and advanced stages factor. In present study, profiles of were examined by immunohistochemistry (IHC) using two anti-MUC4 monoclonal antibodies (MAbs), 8G7 1G8, anti-MUC1 MAb DF3 104 gastrectomy specimens adenocarcinoma with submucosal invasion (pT1b2), 197 histological subtype lesions. Before IHC human specimens, we evaluated specificity MAbs Western blotting mRNA expressing cancer cell lines. reacted clearly, whereas 1G8 did not show any reactivity, either or IHC. rates MUC4/8G7 detected 8G7, MUC4/1G8 MUC1/DF3 well differentiated types (70%, 38/54; 67%, 36/54; 52%, 28/54) significantly higher than those poorly (18%, 10/55; 36%, 20/55; 13%, 7/55) (P<0.0001; P = 0.0021; P<0.0001), respectively. The was related lymphatic (r 0.304, 0.033). On other hand, 0.395, 0.001) lymph node metastasis 0.296, 0.045). 0.357, 0.032) venous 0.377, 0.024). conclusion, as cancers useful marker to predict factors vessel invasion.