Successive Observation of Growth and Movement of Genetically lux-marked Pseudomonas cichorii and the Response of Host Tissues in the Same Lettuce Leaf

摘要: Pseudomonas cichorii SPC9001 was transformed with plasmid pNP126 containing luxCDABE operon of Vibrio fisheri, and bioluminescent IBRC82 obtained. Growth movement the response host tissue were successively observed in same lettuce leaf using video-intensified microscopy (VIM) camera equipped ARGUS 50. Bacterial bioluminescence conferred by lux first at inoculated sites 2 days after inoculation then surrounding region. It moved along veins. Browning symptoms appeared areas where had been detected developed into bacterial rot. Microscopic observation showed that P. colonized intercellular spaces epidermis mesophyll vascular bundle. These results suggest disease symptom appears develops, following growth a leaf. In disks infiltrated bacteria, whole 12hr inoculation, collapsed 36hr inoculation. Cycloheximide, eukaryote-specific protein synthesis inhibitor, completely suppressed collapse disks, but no effect on bioluminescence. demonstrate requires Therefore, develops depending tissues, including de novo synthesis, corresponding to