TREX1 acts in degrading damaged DNA from drug-treated tumor cells.
作者： Chuan-Jen Wang , Wing Lam , Scott Bussom , Hua-Mei Chang , Yung-Chi Cheng
DOI: 10.1016/J.DNAREP.2009.06.006
关键词: Clonogenic assay 、 Proliferating cell nuclear antigen 、 Camptothecin 、 DNA damage 、 Cell biology 、 Molecular biology 、 Cell culture 、 Biology 、 DNA 、 Apoptosis 、 DNA repair
摘要: The major mammalian exonuclease TREX1 has been proposed to play a role in DNA repair and drug resistance. However, no cellular evidence substantiates this claim. Recent reports indicate TREX1's involvement autoimmunity. To further understand its role, we studied expression functionality anticancer drug-treated tumor cells. We report that the localization of are cell-type dependent. Camptothecin other damaging agents induced both protein mRNA dose- time-dependent manner. Using TREX1-inducible cell line, performed clonogenic assays found change sensitivity cells upon induction, suggesting may not or sensitivity. Nevertheless, serves as key enzyme degradation from dying leading less DNA. Ubiquitously expressed normal tissues, act degrading all types undergoing process before phagocytosis occurs.
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sci-hub.se 参考文章(56)
Jozef Laurincik, Poul Maddox-Hyttel, Nudeolar Remodeling in Nuclear Transfer Embryos Advances in Experimental Medicine and Biology. ,vol. 591, pp. 84- 92 ,(2007) , 10.1007/978-0-387-37754-4_6
Anne Krug, Nucleic acid recognition receptors in autoimmunity. Handbook of experimental pharmacology. ,vol. 183, pp. 129- 151 ,(2008) , 10.1007/978-3-540-72167-3_7
F W Perrino, H Miller, K A Ealey, Identification of a 3'-->5'-exonuclease that removes cytosine arabinoside monophosphate from 3' termini of DNA. Journal of Biological Chemistry. ,vol. 269, pp. 16357- 16363 ,(1994) , 10.1016/S0021-9258(17)34015-2
Y H Hsiang, R Hertzberg, S Hecht, L F Liu, Camptothecin induces protein-linked DNA breaks via mammalian DNA topoisomerase I. Journal of Biological Chemistry. ,vol. 260, pp. 14873- 14878 ,(1985) , 10.1016/S0021-9258(17)38654-4
Felix R. Althaus, Hanna E. Kleczkowska, Maria Malanga, Cedric R. Müntener, Jutta M. Pleschke, Maria Ebner, Bernhard Auer, Poly ADP-ribosylation: a DNA break signal mechanism. Molecular and Cellular Biochemistry. ,vol. 193, pp. 5- 11 ,(1999) , 10.1007/978-1-4419-8740-2_1
T Lindahl, J A Gally, G M Edelman, Properties of Deoxyribonuclease III from Mammalian Tissues Journal of Biological Chemistry. ,vol. 244, pp. 5014- 5019 ,(1969) , 10.1016/S0021-9258(18)94303-6
Udo S. Gaipl, Reinhard E. Voll, Ahmed Sheriff, Sandra Franz, Joachim R. Kalden, Martin Herrmann, Impaired clearance of dying cells in systemic lupus erythematosus Autoimmunity Reviews. ,vol. 4, pp. 189- 194 ,(2005) , 10.1016/J.AUTREV.2004.10.007
Dipanjan Chowdhury, Paul J. Beresford, Pengcheng Zhu, Dong Zhang, Jung-Suk Sung, Bruce Demple, Fred W. Perrino, Judy Lieberman, The exonuclease TREX1 is in the SET complex and acts in concert with NM23-H1 to degrade DNA during granzyme A-mediated cell death. Molecular Cell. ,vol. 23, pp. 133- 142 ,(2006) , 10.1016/J.MOLCEL.2006.06.005
Yun-Gui Yang, Tomas Lindahl, Deborah E. Barnes, Trex1 Exonuclease Degrades ssDNA to Prevent Chronic Checkpoint Activation and Autoimmune Disease Cell. ,vol. 131, pp. 873- 886 ,(2007) , 10.1016/J.CELL.2007.10.017
Dan J. Mazur, Fred W. Perrino, Structure and Expression of the TREX1 and TREX2 3′→5′ Exonuclease Genes Journal of Biological Chemistry. ,vol. 276, pp. 14718- 14727 ,(2001) , 10.1074/JBC.M010051200