Taurine prevents the ischemia-induced apoptosis in cultured neonatal rat cardiomyocytes through Akt/caspase-9 pathway.
作者： Tomoka Takatani , Kyoko Takahashi , Yoriko Uozumi , Takahisa Matsuda , Takashi Ito
DOI: 10.1016/J.BBRC.2004.02.066
关键词: Myocyte 、 Cytochrome c 、 Apoptosis 、 Endocrinology 、 Biology 、 Signal transduction 、 Caspase 、 Cell biology 、 Caspase-9 、 Taurine 、 Internal medicine 、 Protein kinase B
摘要: Activated Akt kinase has been proposed as a central role in suppressing apoptosis by modulating the activities of Bcl-2 family proteins and/or caspase-9. To study mechanism underlying anti-apoptotic effect taurine, interaction between taurine and Akt/caspase-9 pathway was examined using simulated ischemia model with cultured rat neonatal cardiomyocytes sealed closed flasks. Taurine (20mM) treatment attenuated ischemia-induced decline activity Akt. Although had no on expression mitochondria level cytosolic cytochrome c, it inhibited cleavage caspases 9 3. Moreover, adenovirus transfer dominant negative form objected taurine-mediated effects, cancelling suppression caspase-9 caspase-3 taurine. These findings provide first evidence that inhibits cardiac myocytes increase activities, inactivating
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