Influence of Analyte Concentration on Stability Constant Values Determined by Capillary Electrophoresis
作者： Viktoria V. Sursyakova , Galina V. Burmakina , Anatoly I. Rubaylo
关键词: Chromatography 、 Analyte 、 Capillary electrophoresis 、 Electrolyte 、 Analytical chemistry 、 Electrophoresis 、 Stability constants of complexes 、 Capillary action 、 Ligand (biochemistry) 、 Chemistry 、 Kinetics
摘要: The influence of analyte concentration when compared with the a charged ligand in background electrolyte (BGE) on measured values electrophoretic mobilities and stability constants (association, binding or formation constants) is studied using capillary electrophoresis (CE) dynamic mathematical simulator CE. study performed labile complexes (with fast kinetics) iron (III) 5-sulfosalicylate ions (ISC) as an example. It shown that because zone not equal to BGE, considerable changes migration times are observed, resulting systematic errors constant values. Of crucial significance slope dependence mobility decrease equilibrium concentration. Without prior information this accurately evaluate for similar systems, total must be at least >50-100 higher than analyte. Experimental ISC peak fronting difference between direction experimental pH simulation allow assuming presence wall interaction.
oup.com
oxfordjournals.org
sci-hub.se 参考文章(36)
Viktoria V. Sursyakova, Anatoly I. Rubaylo, New peak broadening parameter for the characterization of separation capability in capillary electrophoresis. Journal of Separation Science. ,vol. 38, pp. 690- 696 ,(2015) , 10.1002/JSSC.201401109
George W. Gokel, John Aurie Dean, Dean's handbook of organic chemistry ,(2003)
Hadewych Halewyck, Lise Schotte, Iuliana Oita, Bert Thys, Ann Van Eeckhaut, Yvan Vander Heyden, Bart Rombaut, Affinity capillary electrophoresis to evaluate the complex formation between poliovirus and nanobodies Journal of Separation Science. ,vol. 37, pp. 3729- 3737 ,(2014) , 10.1002/JSSC.201400406
Michael C. Breadmore, Hiu Ying Kwan, Jitka Caslavska, Wolfgang Thormann, Dynamic high-resolution computer simulation of electrophoretic enantiomer separations with neutral cyclodextrins as chiral selectors Electrophoresis. ,vol. 33, pp. 958- 969 ,(2012) , 10.1002/ELPS.201100472
Ning Fang, Ying Sun, Jingyan Zheng, David D. Y. Chen, Computer simulation of different modes of ACE based on the dynamic complexation model Electrophoresis. ,vol. 28, pp. 3214- 3222 ,(2007) , 10.1002/ELPS.200600662
Abolghasem Jouyban, Ernst Kenndler, Theoretical and empirical approaches to express the mobility of small ions in capillary electrophoresis. Electrophoresis. ,vol. 27, pp. 992- 1005 ,(2006) , 10.1002/ELPS.200500696
Viktoriya V. Sursyakova, Sergey N. Kalyakin, Galina V. Burmakina, Anatoliy I. Rubaylo, System peaks in capillary zone electrophoresis of anions with negative voltage polarity and counter-electroosmotic flow ELECTROPHORESIS. ,vol. 32, pp. 210- 217 ,(2011) , 10.1002/ELPS.201000313
V. V. Sursyakova, S. N. Kalyakin, G. V. Burmakina, A. I. Rubaylo, System peaks and optimization of anion separation in capillary electrophoresis with non-reversed electroosmotic flow Journal of Analytical Chemistry. ,vol. 67, pp. 783- 789 ,(2012) , 10.1134/S1061934812090080
Wolfgang Thormann, Jitka Caslavska, Michael C. Breadmore, Richard A. Mosher, Dynamic computer simulations of electrophoresis: three decades of active research. Electrophoresis. ,vol. 30, ,(2009) , 10.1002/ELPS.200900058
Zhi Chen, Stephen G. Weber, Determination of binding constants by affinity capillary electrophoresis, electrospray ionization mass spectrometry and phase-distribution methods. Trends in Analytical Chemistry. ,vol. 27, pp. 738- 748 ,(2008) , 10.1016/J.TRAC.2008.06.008