New peak broadening parameter for the characterization of separation capability in capillary electrophoresis.
作者： Viktoria V. Sursyakova , Anatoly I. Rubaylo
关键词:
摘要: The influence of separation conditions on peak broadening is usually estimated by the number theoretical plates. Using data available in literature and experimental data, it shown that pressure-assisted capillary electrophoresis plate not directly related to capability used. experiments at different electrolyte flow velocities demonstrate a higher (the best efficiency) can be obtained with lower resolution. Since ions are separated due difference electrophoretic mobilities, width terms mobility suggested as new parameter describing calculated using tailing factor temporal 5% height. A simple equation for resolution calculation derived parameter. advantage over other parameters shown. recommended used only but also general when runs virtual separative migration distance change widely.
sci-hub.se 参考文章(45)
Petr Tůma, Rapid determination of globin chains in red blood cells by capillary electrophoresis using INSTCoated fused-silica capillary. Journal of Separation Science. ,vol. 37, pp. 1026- 1032 ,(2014) , 10.1002/JSSC.201400044
Pavel Řezanka, Lenka Rokosová, Klára Řezanková, Markéta Bláhová, Michal Řezanka, David Sýkora, Jindřich Jindřich, Vladimír Král, The influence of the substituent position in monocarboxymethyl‐γ‐cyclodextrins on enantioselectivity in capillary electrophoresis Journal of Separation Science. ,vol. 37, pp. 2779- 2784 ,(2014) , 10.1002/JSSC.201400604
Lin Tan, Xiajun Zheng, Lijuan Chen, Yanmei Wang, Quality testing of human albumin by capillary electrophoresis using thermally cross-linked poly(vinyl pyrrolidone)-coated fused-silica capillary. Journal of Separation Science. ,vol. 37, pp. 2974- 2982 ,(2014) , 10.1002/JSSC.201400463
Joanna Witos, Mikko Karesoja, Erno Karjalainen, Heikki Tenhu, Marja-Liisa Riekkola, Surface initiated polymerization of a cationic monomer on inner surfaces of silica capillaries: Analyte separation by capillary electrophoresis versus polyelectrolyte behavior Journal of Separation Science. ,vol. 36, pp. 1070- 1077 ,(2013) , 10.1002/JSSC.201200945
Hong-Mei Hu, Xue-Feng Yin, Xiu-Zhong Wang, Hong Shen, A study on the system of nonaqueous microchip electrophoresis with on-line peroxyoxalate chemiluminescence detection. Journal of Separation Science. ,vol. 36, pp. 713- 720 ,(2013) , 10.1002/JSSC.201200832
Purnendu K. Dasgupta, Satyajit Kar, Effects of separation potential, hydrostatic pressure and auxiliary electroosmotic pumping on a suppressed conductometric capillary electrophoresis separation system Analytica Chimica Acta. ,vol. 394, pp. 1- 12 ,(1999) , 10.1016/S0003-2670(99)00209-3
Ernst Kenndler, Dependence of analyte separation on electroosmotic flow in capillary zone electrophoresis: Quantitative description by the reduced mobility Journal of Microcolumn Separations. ,vol. 10, pp. 273- 279 ,(1998) , 10.1002/(SICI)1520-667X(1998)10:3<273::AID-MCS6>3.0.CO;2-D
Zs. Pápai, T.L. Pap, Analysis of peak asymmetry in chromatography. Journal of Chromatography A. ,vol. 953, pp. 31- 38 ,(2002) , 10.1016/S0021-9673(02)00121-8
Viktoriya V. Sursyakova, Sergey N. Kalyakin, Galina V. Burmakina, Anatoliy I. Rubaylo, System peaks in capillary zone electrophoresis of anions with negative voltage polarity and counter-electroosmotic flow ELECTROPHORESIS. ,vol. 32, pp. 210- 217 ,(2011) , 10.1002/ELPS.201000313
Christopher T. Culbertson, James W. Jorgenson, Increasing the resolving power of capillary electrophoresis through electroosmotic flow control using radial fields Journal of Microcolumn Separations. ,vol. 11, pp. 167- 174 ,(1999) , 10.1002/(SICI)1520-667X(1999)11:3<167::AID-MCS1>3.0.CO;2-Y