Quality testing of human albumin by capillary electrophoresis using thermally cross-linked poly(vinyl pyrrolidone)-coated fused-silica capillary.

摘要: To detect the quality of medicinal human albumin by capillary electrophoresis, we produced a fused-silica coated with thermally cross-linked poly(vinyl pyrrolidone) to prohibit protein adsorption. This type was easily obtained injecting an aqueous solution into and annealing it at 200°C. Notably, stable low electro-osmotic flow in pyrrolidone)-coated pH 2.20–9.00, separation mixture four basic proteins indicated that exhibits excellent repeatability efficiency; moreover, these could even be achieved 7.00. The recovery percentage serum single-protein mixed blood determined 97.03 95.40% pyrrolidone)50–3 (representing concentration capillary-injected solution, 50 mg/mL, thermal time, 3 h) capillary, respectively. Based on results, used pyrrolidone)50–3-coated quantify content albumin, results from run run, day demonstrated for testing commercially available albumin.