Deregulated microRNAs in CD4+ T cells from individuals with latent tuberculosis versus active tuberculosis

摘要: The mechanisms of latent tuberculosis (TB) infection remain elusive. Roles microRNA (miRNA) have been highlighted in pathogen–host interactions recently. To identify miRNAs involved the immune response to TB, expression profiles CD4+ T cells from patients with active TB and healthy controls were investigated by microarray assay validated RT-qPCR. Gene ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) pathway analysis used analyse significant functions involvement signalling pathways differentially expressed miRNAs. potential target genes for miR-29, interferon-γ (IFN-γ) mRNA was measured Our results showed that 27 deregulated among three groups. RT-qPCR generally consistent data. We observed an inverse correlation between miR-29 level IFN-γ cells. GO KEGG possible significantly enriched mitogen-activated protein kinase pathway, focal adhesion extracellular matrix receptor interaction, which might be transition TB. In all, first time, our study revealed some altered Function miRNA-deregulated mRNAs help improve understanding relationship laid important foundation further identification underlying its reactivation.