Identifcation of differentially expressed long non-coding RNAs in CD4+ T cells response to latent tuberculosis infection.

摘要: Summary Objective To identify differentially expressed long non-coding RNAs (lncRNAs) in CD4 + T cells triggered upon latent tuberculosis (TB) infection. Methods Expression profiles of lncRNAs and mRNAs from individuals with TB infection (LTBI), active healthy controls were analyzed by microarray assay four selected for validation using real time-quantitative polymerase chain reaction (RT-qPCR). Gene ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) pathway based approaches used to investigate biological functions signaling pathways affected the mRNAs. Results LncRNAs involved LTBI disease. Compared controls, 449 461 deregulated group, 1,113 1,490 as well 163 187 both group. It was worth noting that 41 60 between three groups. Most intergenic regions (∼50%), natural antisense protein-coding loci (∼20%), or intronic (∼10%). Significantly enriched on mainly mitogen-activated protein kinase (MAPK) pathway, cytokine–cytokine receptor interaction, Toll-like etc. Conclusions The study first report response indicated some may be regulating host immune Future studies are needed further elucidate potential roles these its reactivation.