Gene marking to determine whether autologous marrow infusion restores long-term haemopoiesis in cancer patients

摘要: Abstract The contribution of infused bone marrow cells to long-term haemopoietic recovery in patients undergoing autologous transplantation is unknown. Such information would help clarify the role this procedure cancer therapy and aid development strategies reduce risk subsequent aplasia. By transferring a neomycin resistance marker gene into 20 before transplantation, we were able trace pattern reconstitution postinfusion. was present expressed all lineages vivo 15 18 evaluable at 1 month post-transplantation, 8 9 6 months, 5 year. has remained detectable for up months—the duration our study. Our findings indicate that harvested consistently contributes multilineage haemopoiesis after patients. These results provide rationale continued exploration more ablative preparative regimens with single or sequential transplants.