Genetic marking shows that Ph+ cells present in autologous transplants of chronic myelogenous leukemia (CML) contribute to relapse after autologous bone marrow in CML.

摘要: Relapse after autologous bone marrow transplantation for chronic myelogenous leukemia (CML) can be due either to the persistence of cells in systemic tissues following preparative therapy, or used restore function intensive therapy. To help distinguish between these two possible causes relapse, we safety-modified retroviruses, which contain bacterial resistance gene NEO, mark that had been collected from patients early phase hematopoietic recovery vivo chemotherapy. The were then subjected ex CD34 selection collection and 30% exposed a virus. This was infused delivery consisted total body irradiation (1,020 cGy), cyclophosphamide (120 mg/kg), VP-16 (750 mg/m2). RT PCR assays specific NEO mRNA, coded by virus, bcr-abl mRNA showed evaluable CML transplanted with marked cells, sufficient numbers remained contribute relapse. In addition, both normal leukemic positive retroviral transgenome persisted circulation at least 280 days posttransplant showing responsible return hematopoiesis observation shows it is use replication-incompetent retrovirus order introduce DNA sequences into undergoing transplantation. Moreover, this data suggested additional fractionation procedures will necessary reduce probability relapse advanced stages disease procedures.