Western-blotting method for detecting antibodies against human muscle contractile proteins in myositis.
作者： Kozaburo Koga , Shigeto Abe , Hiroshi Hashimoto , Masahiro Yamaguchi
DOI: 10.1016/0022-1759(87)90409-1
关键词: Pathology 、 Myosin 、 Troponin complex 、 Tropomyosin 、 Molecular biology 、 Immunoglobulin light chain 、 Blot 、 Antigen 、 Muscular dystrophy 、 Antibody 、 Biology
摘要: Many investigators report anti-muscle antibodies using various kinds of methods. The Western-blotting method, however, has not previously been used for this purpose. We have detected to muscle contractile proteins in sera from patients with collagen disease and muscular dystrophy by method. antigens included myosin heavy light chains, tropomyosin troponin complex. Our method is a quick sensitive way determine which are the antigenic proteins.
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