Identification of alpha- and beta-cardiac myosin heavy chain isoforms as major autoantigens in dilated cardiomyopathy.

摘要: BACKGROUNDImmunization with cardiac myosin induces experimental autoimmune heart disease in genetically predisposed mice. These mice produce heart-specific autoantibodies, some of which are directed against the isoform.METHODS AND RESULTSWe have reported presence circulating autoantibodies 26% patients idiopathic dilated cardiomyopathy (DCM) using indirect immunofluorescence. To identify autoantigen(s) recognized by human disease, we tested, Western blotting, sera from 26 DCM patients, 14 whom were antibody-positive and 12 antibody-negative, as well failure ischemic or valvular 13 normal subjects who antibody-negative. Crude myofibrillar proteins preparations extracted atrial ventricular specimens used antigens. Sodium dodecyl sulfate polyacrylamide gel electrophoresis was performed. The w...