Myosin autoantibodies detected by immunofluorescence.

摘要: Autoantibodies to striated and smooth muscles myosins were detected by indirect immunofluorescence confirmed absorption with purified contractile proteins extracted from human, rabbit chicken muscle. Myosin antibodies rare: of fifty-five sera examined patients various skeletal cardiac muscle disorders, only one serum, a case Coxsackie viral pericarditis, had anti-myosin activity. It reacted type 1 fibres muscle, staining the 'A' band sarcomere only. The antibody was absorbed myosin heavy meromyosin fragments, but not myosin. Two types autoantibodies are described. One is restricted examples found in polyclonal monoclonal SMA sera. second cross-reacted cytoplasmic liver, kidney thyroid cells. completely using either or fragments. different reflect variety mammalian tissues. Cross-reacting indicate epitopes on fragment which common several tissue myosins.