Cardiac myosin-induced myocarditis. Heart autoantibodies are not involved in the induction of the disease.

摘要: We recently demonstrated that cardiac myosin is capable of inducing autoimmune myocarditis in genetically predisposed mice. This disease parallels coxsackievirus B3-induced many respects and associated with high-titer autoantibodies specific for myosin. The following lines evidence suggest these are not involved the induction myocarditis: 1) immunoperoxidase staining heart sections from myosin-immunized A/J A.SW mice revealed IgG depositions only along damaged muscle fibres infiltrated areas, but intact tissue; 2) did bind to surface viable myocytes isolated mice, reacted permeabilized detergent; 3) treated a single high dose cyclophosphamide, which reduces humoral immune response, still developed severe myocarditis, despite fact their autoantibody titers were reduced level adjuvant-injected controls; 4) passive transfer failed induce although recipients comparable those found myosin-induced disease. Together, results secondary rather than primary