A quencher-free molecular beacon design based on pyrene excimer fluorescence using pyrene-labeled UNA (unlocked nucleic acid)
作者： Kasper K. Karlsen , Anders Okholm , Jørgen Kjems , Jesper Wengel
DOI: 10.1016/J.BMC.2013.04.062
关键词: Fluorescence 、 Excimer 、 Pyrene 、 Monomer 、 Photochemistry 、 Complementary rna 、 Molecular beacon 、 RNA 、 Nucleic acid 、 Chemistry 、 Organic chemistry 、 Clinical biochemistry 、 Molecular medicine 、 Biochemistry 、 Molecular biology 、 Drug discovery 、 Pharmaceutical Science
摘要: A quencher-free molecular beacon capable of generating pyrene excimer fluorescence has been constructed using strategically positioned pyrene-UNA monomers. Hybridization a fully complementary RNA target was accompanied by emission increase more than 900%, and detection in living cells demonstrated.
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B. Deiman, C. Schrover, C. Moore, D. Westmoreland, P. van de Wiel, Rapid and highly sensitive qualitative real-time assay for detection of respiratory syncytial virus A and B using NASBA and molecular beacon technology Journal of Virological Methods. ,vol. 146, pp. 29- 35 ,(2007) , 10.1016/J.JVIROMET.2007.05.030
Dmitry M. Kolpashchikov, Binary probes for nucleic acid analysis. Chemical Reviews. ,vol. 110, pp. 4709- 4723 ,(2010) , 10.1021/CR900323B
Patrick Conlon, Chaoyong James Yang, Yanrong Wu, Yan Chen, Karen Martinez, Youngmi Kim, Nathan Stevens, Angel A. Marti, Steffen Jockusch, Nicholas J. Turro, Weihong Tan, Pyrene Excimer Signaling Molecular Beacons for Probing Nucleic Acids Journal of the American Chemical Society. ,vol. 130, pp. 336- 342 ,(2008) , 10.1021/JA076411Y
Gil Tae Hwang, Young Jun Seo, Byeang Hyean Kim, A Highly Discriminating Quencher-Free Molecular Beacon for Probing DNA Journal of the American Chemical Society. ,vol. 126, pp. 6528- 6529 ,(2004) , 10.1021/JA049795Q
Francoise M. Winnik, PHOTOPHYSICS OF PREASSOCIATED PYRENES IN AQUEOUS POLYMER SOLUTIONS AND IN OTHER ORGANIZED MEDIA Chemical Reviews. ,vol. 93, pp. 587- 614 ,(1993) , 10.1021/CR00018A001
Kasper K. Karlsen, Troels B. Jensen, Jesper Wengel, Synthesis of an unlocked nucleic acid terpyridine monomer and binding of divalent metal ion in nucleic acid duplexes. Journal of Organic Chemistry. ,vol. 74, pp. 8838- 8841 ,(2009) , 10.1021/JO9019978
Hiromu Kashida, Kyohei Yamaguchi, Yuichi Hara, Hiroyuki Asanuma, Quencher-free molecular beacon tethering 7-hydroxycoumarin detects targets through protonation/deprotonation. Bioorganic & Medicinal Chemistry. ,vol. 20, pp. 4310- 4315 ,(2012) , 10.1016/J.BMC.2012.05.052
L. Stryer, R. P. Haugland, Energy transfer: a spectroscopic ruler. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 58, pp. 719- 726 ,(1967) , 10.1073/PNAS.58.2.719
Sarah M. Biner, Dominic Kummer, Vladimir L. Malinovskii, Robert Häner, Signal control by self-assembly of fluorophores in a molecular beacon—a model study Organic and Biomolecular Chemistry. ,vol. 9, pp. 2628- 2633 ,(2011) , 10.1039/C0OB01132K
Michael Petersen, Jesper Wengel, LNA: a versatile tool for therapeutics and genomics Trends in Biotechnology. ,vol. 21, pp. 74- 81 ,(2003) , 10.1016/S0167-7799(02)00038-0