PNA Molecular Beacons Assembled by Post-Synthetic Click Chemistry Functionalization.

摘要: To avoid the tedious synthesis of functionalized peptide nucleic acid (PNA) monomers for probe development, we proposed a simple approach to modify PNA oligomers by post-synthetic on-resin click chemistry. molecular beacons (MBs) were prepared incorporation azide-containing into oligomer automatic solid-phase and subsequent derivatization with pyrene moieties copper-catalyzed azide-alkyne cycloaddition. Two pyrene-based quencher-free beacons, stemless MB one possessing stem-loop structure, targeting portion cystic fibrosis gene, successfully synthesized using this method. Fluorescence studies showed that exhibited better discrimination changes in excimer/monomer ratios as compared construct.