Rapid and highly sensitive qualitative real-time assay for detection of respiratory syncytial virus A and B using NASBA and molecular beacon technology
作者： B. Deiman , C. Schrover , C. Moore , D. Westmoreland , P. van de Wiel
DOI: 10.1016/J.JVIROMET.2007.05.030
关键词:
摘要: The performance of a sensitive and specific qualitative respiratory syncytial virus (RSV) assay based on NASBA technology real-time molecular beacon detection is presented. Very low limits for both RSV A B were determined: 95% hit-rate 95 47 copies/input in isolation B, respectively. was detected wide variety clinical samples including swabs, nasopharyngeal aspirates (NPA), bronchoalveolar lavages (BAL), endotracheal secretions, sputum samples. In total 779 tested valid result obtained 765 (RSV assay), (cell culture), 529 (rapid direct immunofluorescence testing (IF)) Of these samples, 229 61 122 (IF) positive RSV. addition, 106 reported as negative using the NOW® (Binax). Subsequent demonstrated that 32 (30%) positive. includes homologous internal control, which offers high degree standardization quality control. When performed NucliSens EasyQ platform (bioMerieux), test results 48 sample extracts less than 2 h.
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sci-hub.se 参考文章(16)
G Dewhurst-Maridor, V Simonet, J.E Bornand, L.P Nicod, J.C Pache, Development of a quantitative TaqMan RT-PCR for respiratory syncytial virus Journal of Virological Methods. ,vol. 120, pp. 41- 49 ,(2004) , 10.1016/J.JVIROMET.2004.03.017
Birgit Deiman, Pierre van Aarle, Peter Sillekens, Characteristics and applications of nucleic acid sequence-based amplification (NASBA). Molecular Biotechnology. ,vol. 20, pp. 163- 179 ,(2002) , 10.1385/MB:20:2:163
P. Gruteke, A. S. Glas, M. Dierdorp, W. B. Vreede, J.-W. Pilon, S. M. Bruisten, Practical Implementation of a Multiplex PCR for Acute Respiratory Tract Infections in Children Journal of Clinical Microbiology. ,vol. 42, pp. 5596- 5603 ,(2004) , 10.1128/JCM.42.12.5596-5603.2004
Wade K Aldous, Kris Gerber, Edward W Taggart, Joshua Thomas, David Tidwell, Judy A Daly, A comparison of binax™ now® to viral culture and direct fluorescent assay testing for respiratory syncytial virus Diagnostic Microbiology and Infectious Disease. ,vol. 49, pp. 265- 268 ,(2004) , 10.1016/J.DIAGMICROBIO.2004.04.005
A. R. Falsey, M. A. Formica, E. E. Walsh, Diagnosis of Respiratory Syncytial Virus Infection: Comparison of Reverse Transcription-PCR to Viral Culture and Serology in Adults with Respiratory Illness Journal of Clinical Microbiology. ,vol. 40, pp. 817- 820 ,(2002) , 10.1128/JCM.40.3.817-820.2002
L. Liolios, A. Jenney, D. Spelman, T. Kotsimbos, M. Catton, S. Wesselingh, Comparison of a Multiplex Reverse Transcription-PCR-Enzyme Hybridization Assay with Conventional Viral Culture and Immunofluorescence Techniques for the Detection of Seven Viral Respiratory Pathogens Journal of Clinical Microbiology. ,vol. 39, pp. 2779- 2783 ,(2001) , 10.1128/JCM.39.8.2779-2783.2001
M. J. Ohm-Smith, P. S. Nassos, B. L. Haller, Evaluation of the Binax NOW, BD Directigen, and BD Directigen EZ Assays for Detection of Respiratory Syncytial Virus Journal of Clinical Microbiology. ,vol. 42, pp. 2996- 2999 ,(2004) , 10.1128/JCM.42.7.2996-2999.2004
D. M. Whiley, M. W. Syrmis, I. M. Mackay, T. P. Sloots, Detection of Human Respiratory Syncytial Virus in Respiratory Samples by LightCycler Reverse Transcriptase PCR Journal of Clinical Microbiology. ,vol. 40, pp. 4418- 4422 ,(2002) , 10.1128/JCM.40.12.4418-4422.2002
C. Moore, M. Valappil, S. Corden, D. Westmoreland, Enhanced clinical utility of the NucliSens EasyQ RSV A+B Assay for rapid detection of respiratory syncytial virus in clinical samples European Journal of Clinical Microbiology & Infectious Diseases. ,vol. 25, pp. 167- 174 ,(2006) , 10.1007/S10096-006-0112-4
A. Hu, M. Colella, J. S. Tam, R. Rappaport, S.-M. Cheng, Simultaneous detection, subgrouping, and quantitation of respiratory syncytial virus A and B by real-time PCR. Journal of Clinical Microbiology. ,vol. 41, pp. 149- 154 ,(2003) , 10.1128/JCM.41.1.149-154.2003