Simultaneous detection, subgrouping, and quantitation of respiratory syncytial virus A and B by real-time PCR.

摘要: Timely diagnosis of respiratory syncytial virus (RSV) infection is critical for appropriate treatment lower in young children. To facilitate diagnosis, we developed a rapid, specific, and sensitive TaqMan PCR method detection RSV A B. Two sets primer-probe pairs were selected from the nucleotide sequences encoding nucleocapsid protein—one targeting other The specificity reverse transcription-PCR assay was evaluated by testing each pair against various viruses derived laboratory stocks, as well clinical specimens. Fluorescent signals observed only presence and/or sensitivity our quantitative determined on basis PFU particle counts. resulting found to be 0.023 PFU, or two copies viral RNA, 0.018 nine This utilized diagnose 175 nasopharyngeal aspirates obtained children Hong Kong with symptoms during winter 2000 2001. Among these specimens, detected 36 RSV-positive samples, 10 which identified 26 B, whereas culture confirmation 21 specimens immunofluorescence 32 all among those PCR. results confirmed accuracy demonstrated its improved versus classical methods.