Clinical evaluation of NucliSENS magnetic extraction and NucliSENS analytical specific reagents for the real-time detection of respiratory syncytial virus (RSV) in paediatric respiratory specimens

摘要: Aims: To evaluate the combination of NucliSENS magnetic extraction and analytic specific reagents (bioMerieux, Marcy L’Etoile, France) for detection respiratory syncytial virus (RSV) from a variety samples. Methods: Nucleic acids (NA) pediatric samples (n=603) RSV inhibition control (R-IC) were co-extracted using miniMAG and/or easyMAG. acid sequenced based amplification (NASBA) molecular beacon R-IC performed ASRs (NRSVA) EasyQ Analyzer. NRSVA results compared to R-Mix culture direct fluorescent antibody (DFA). Results: analytical specificity was 100%. limit 5-20 RNA copies/reaction. Pre discordant analysis, sensitivity, specificity, PPV NPV were, respectively, (64.7%, 100%, 94.5%); DFA (98.8%, 99.0%, 94.4%, 99.8%); (94.1%, 95%, 75.5%, 99%). After (56.7%, 92.3%); (87.6%, 99.2%, 95.5%, 97.7%); (93.8%, 97%, 85.9%, 98.8%). detected in 17.8% 7 co-infections. Children with proven infection, children without pathogen identified, had shorter median hospitalization stays (2 days versus 3 days, P=0.035), used less antibiotics (54% 69%) durations antibiotic therapy (6.2 9.3 P=0.021), respectively. Conclusions: is sensitive The monitored test process, including NA extraction, target detection. rapid pathogens can foster appropriate patient management.