Crkl is the major tyrosine-phosphorylated protein in neutrophils from patients with chronic myelogenous leukemia.

摘要: The Philadelphia chromosome (Ph1), detected in virtually all cases of chronic myelogenous leukemia (CML), is formed by a reciprocal translocation between 9 and 22 that fuses Bcr-encoded sequences upstream exon 2 c-Abl. This oncogene produces fusion protein, p210bcr-abl, which the Abl tyrosine kinase activity elevated. Using anti-phosphotyrosine immunoblotting, we have compared pattern phosphotyrosine-containing proteins from freshly prepared neutrophils patients stable phase CML to normal controls. only consistent difference was presence 39-kDa tyrosine-phosphorylated protein 18 out neutrophil samples not seen same as assessed two-dimensional also present cell lines expressing including K562 cells. cells source purified identified microsequencing Crkl, an SH2/SH3 adaptor related crk avian sarcoma virus, CT10. A direct interaction Crkl has been shown using yeast two-hybrid screen.