Preparation of guluronate lyase from Pseudomonas alginovora for protoplast isolation in Laminaria
作者： C. Boyen , Y. Bertheau , T. Barbeyron , B. Kloareg
DOI: 10.1016/0141-0229(90)90027-N
关键词: Laminaria digitata 、 Size-exclusion chromatography 、 Ammonium 、 Pseudomonadales 、 Biochemistry 、 Laminaria 、 Protoplast 、 Pseudomonadaceae 、 Lyase 、 Biology 、 Biotechnology 、 Applied Microbiology and Biotechnology
摘要: Abstract A marine aerobic bacterium identified as Pseudomonas alginovora was isolated from decaying fronds of Laminaria digitata. It secreted an endo- acting alginate lyase the guluronate-specific type. The enzyme purified to homogeneity in SDS-PAGE by precipitation with ammonium sulphate, gel filtration, and anion-exchange chromatography. fractions were effective producing protoplasts species .
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