Development of a fermentation process for production of an alginate G-lyase from Klebsiella pneumoniae

摘要: A high-density-cell fermentation process for production of an exracellular alginat lyase from Klebseilla pneumoniae on a defined medium has been developed. The employs strategy using two carbon sources. One low-molecular-mass, low-viscosity source (sucrose) with high water solubililty is used as the main carbons growth, while high-molecular-mass and viscoous alginate in low concentration inducer enzyme synthesis. repression algiante by sucrose growth inhibition that we observed at increased levels ammonia were circumvented computer-assisted fed-batch addition sources (succrose alginate) supplying nitrogen pH control. No was when dissolved oxygen limited uptake rate 40%–50% maximum rate. An optimal composition feeding solution (12.5 g 587.5 1−1) found both final (1330 U volumetric (67 1−1 h−1). dependes linear phase, giving highest tested. shows fiveflod increase compare previously reproted daata where source. In addition, ratio factor apporximately 15. doubling extracellular specific activity observed, property significant interest, especially purification enzyme. On othr hand, dry cell weight bacteria also 15–20 thus relatively lower productivity 0.4 (g weight)−1 h−1.