Enzymatic microassay for the determination and characterization of alginates

摘要: Abstract When alginate is split by an lyase, unsaturated unit with a strong absorbance at 230 nm formed the new non-reducing end. At excessive enzyme levels, will approach apparent endpoint level that reflects initial substrate concentration. On this basis, simple and rapid assay has been developed to detect quantify alginates in solution 0·01–1 mg/ml concentration range. A combination of purified guluronate lyase from Klebsiella pneumoniae mannuronate Haliotis tuberculata applied eliminate influence specific composition alginates. Applied separately combination, these enzymes may also give information about structure accordance their block distribution determined NMR.