Development of a new procedure based on the energy charge measurement using ATP bioluminescence assay for the detection of living mould from graphic documents

摘要: Fungal contamination is a major cause of deterioration in libraries and archives. Curators conservators increasingly need rapid microbiological analyses. This paper presents detection method for the fungal contaminants on documents. A previous study showed that calculation energy charge, using bioluminescence ATP assays, provides useful indicator to determinate viability strains. We argue this sensitive time-saving better than traditional culture techniques. However, procedure needs be modified make it usable lay persons. An improved simplified protocol proposed here extraction adenylate nucleotides (AN) from spores their measurements. Our new can detect existence viable strains documents, presenting suspect spots within minutes. The performed by filtration with DMSO–TE solution as extractant. different step measurement AN content carried out successively single test tube instead three tubes necessary initial method. was tested 12 among those most frequently found archives validated swab samples real Copyright © 2008 John Wiley & Sons, Ltd.