Assessing the microbiological risk to stored sixteenth century parchment manuscripts: a holistic approach based on molecular and environmental studies

摘要: The microbial risk for the conservation of seven sixteenth century parchment manuscripts, which showed brown discolouration putatively caused by microorganisms, was evaluated using non-invasive sampling techniques, microscopy, studies surface-associated and airborne microflora with culture-independent molecular methods, measuring repository thermo-hygrometric values. Microscopic observations ATP assays demonstrated a low level contamination, indicating that not related to currently active colonisation. Nevertheless, approach adopted fully characterise communities searching biodeteriogens could grow under appropriate conditions. Indeed, potential microorganisms are ecologically humans were found, suggesting need control environment improve handling procedures. Microbial loads air measu...