[8] Biomass and adenylate energy charge determination in microbial cell extracts and environmental samples

摘要: Publisher Summary This chapter describes the methodology for quantitative determinations of adenosine triphosphate (ATP), diphosphate (ADP), and monophosphate (AMP) in cell extracts environmental samples, by use firefly luciferin–luciferase bioluminescent reaction. Difficulties sources error are discussed regard to analytical phase as well preliminary steps sampling extraction nucleotides from living cells. Firefly luciferase is specific ATP, hence it not necessary separate ATP other extracts. The rationale using estimate biomass ubiquitous distribution all cells, rapid loss dead fairly uniform concentration protoplasm microbial Data on concentrations can thus be extrapolated parameters.