Shiga toxin and beta-lactamases genes in Escherichia coli phylotypes isolated from carcasses of broiler chickens slaughtered in Iran.
作者： Mahboube Bagheri , Reza Ghanbarpour , Hesam Alizade
DOI: 10.1016/J.IJFOODMICRO.2014.02.003
关键词: Beta-lactamase 、 STX2 、 Shiga toxin 、 Escherichia coli 、 Phylogenetic tree 、 Biology 、 Intimin 、 Phylogenetics 、 Phylotype 、 Microbiology
摘要: Two hundred and four Escherichia coli strains were isolated from external visceral cavity surfaces of 102 slaughtered broiler carcasses. The isolates screened to determine the phylogenetic background presence Shiga toxins (stx1, stx2), intimin (eae) beta-lactamase (blaTEM, blaSHV) genes. Phylotyping results revealed that E. segregated in groups A (56.86%), B1 (19.12%), B2 (4.90%) D (19.12%). PCR assays 13 (6.37%) 12 carcasses positive for eae (12 isolates) and/or stx2 (2) belonged (A0, A1), B1, (B22) (D2). seven recovered surface, whereas only 5 surface On other hand, thirty one 26 carried blaTEM (27) blaSHV (4) genes different phylo-groups. This study suggests could be considered as an important source EPEC STEC pathotypes southeast Iran; well examined antibiotic resistance genes, which by some transferred pathogens through food chain.
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