A cotton LIM domain-containing protein (GhWLIM5) is involved in bundling actin filaments.
作者： Yang Li , Jia Jiang , Lan Li , Xiu-Lan Wang , Na-Na Wang
DOI: 10.1016/J.PLAPHY.2013.01.018
关键词: Cell biology 、 Regulation of gene expression 、 Biology 、 Cytoskeleton 、 Fusion gene 、 Green fluorescent protein 、 LIM domain 、 Plasma protein binding 、 Peptide sequence 、 Actin
摘要: LIM-domain proteins play important roles in cellular processes eukaryotes. In this study, a LIM protein gene, GhWLIM5, was identified cotton. Quantitative RT-PCR analysis showed that GhWLIM5 expressed widely different cotton tissues and had peak expression during fiber elongation. GFP fluorescence assay revealed cells expressing GhWLIM5:eGFP fusion gene displayed network distribution of eGFP fluorescence, suggesting is mainly localized to the cell cytoskeleton. When transformed were stained with rhodamine-phalloidin there consistent overlap rhodamine-palloidin signals, demonstrating colocalized F-actin addition, high-speed cosedimentation verified directly bound actin filaments, while low microscopic observation indicated bundled vitro. Increasing amounts able protect from depolymerization vitro presence Lat B (an depolymerizer). Our results contribute better understanding biochemical role modulating dynamic
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sci-hub.se 参考文章(46)
N Mimura, A Asano, Isolation and characterization of a conserved actin-binding domain from rat hepatic actinogelin, rat skeletal muscle, and chicken gizzard alpha-actinins. Journal of Biological Chemistry. ,vol. 261, pp. 10680- 10687 ,(1986) , 10.1016/S0021-9258(18)67439-3
H CHEN, B BERNSTEIN, J BAMBURG, Regulating actin-filament dynamics in vivo Trends in Biochemical Sciences. ,vol. 25, pp. 19- 23 ,(2000) , 10.1016/S0968-0004(99)01511-X
Joshua P. Thaler, Soo-Kyung Lee, Linda W. Jurata, Gordon N. Gill, Samuel L. Pfaff, LIM factor Lhx3 contributes to the specification of motor neuron and interneuron identity through cell-type-specific protein-protein interactions Cell. ,vol. 110, pp. 237- 249 ,(2002) , 10.1016/S0092-8674(02)00823-1
Marleen Van Troys, Lynn Huyck, Shirley Leyman, Stien Dhaese, Joël Vandekerkhove, Christophe Ampe, Ins and outs of ADF/cofilin activity and regulation European Journal of Cell Biology. ,vol. 87, pp. 649- 667 ,(2008) , 10.1016/J.EJCB.2008.04.001
Steven J Winder, Kathryn R Ayscough, Actin-binding proteins Journal of Cell Science. ,vol. 118, pp. 651- 654 ,(2005) , 10.1242/JCS.01670
S Arber, P Caroni, Specificity of single LIM motifs in targeting and LIM/LIM interactions in situ. Genes & Development. ,vol. 10, pp. 289- 300 ,(1996) , 10.1101/GAD.10.3.289
H. Quader, W. Herth, U. Ryser, E. Schnepf, Cytoskeletal elements in cotton seed hair development in vitro: their possible regulatory role in cell wall organization Protoplasma. ,vol. 137, pp. 56- 62 ,(1987) , 10.1007/BF01281176
Christopher J Staiger, Laurent Blanchoin, Actin dynamics: old friends with new stories. Current Opinion in Plant Biology. ,vol. 9, pp. 554- 562 ,(2006) , 10.1016/J.PBI.2006.09.013
David W. McCurdy, David R. Kovar, Christopher J. Staiger, Actin and actin-binding proteins in higher plants Protoplasma. ,vol. 215, pp. 89- 104 ,(2001) , 10.1007/BF01280306
Julie L. Kadrmas, Mary C. Beckerle, The LIM domain: from the cytoskeleton to the nucleus Nature Reviews Molecular Cell Biology. ,vol. 5, pp. 920- 931 ,(2004) , 10.1038/NRM1499