A rapid assay for drug sensitivity of glioblastoma stem cells
作者： Hilah Gal , Arik Makovitzki , Ninette Amariglio , Gideon Rechavi , Zvi Ram
DOI: 10.1016/J.BBRC.2007.05.020
关键词: Pharmacology 、 Neurosphere 、 Imatinib mesylate 、 Cancer research 、 Biology 、 Programmed cell death 、 Cell culture 、 Stem cell 、 Antigen 、 Drug 、 Immunohistochemistry 、 Biophysics 、 Cell biology 、 Biochemistry 、 Molecular biology
摘要: Glioblastoma (GBM) is a highly infiltrating, aggressive brain cancer with no available curative treatment. We developed rapid assay for assessing the effect of various drugs on GBM stem cells. The uses small number separated CD133+ cells (20,000 in 0.2 ml) 96-well plate that form neurospheres within 1-2 days. Various disperse 24-36 h, which can be quantified microscopically. used cell line A-172 to develop conditions assay, utilizing Gleevec, gamma-secretase inhibitor DAPT, and anti-bacterial peptide amph1D. results show dispersion leading death, at relatively low concentrations (<25 microM). Drug combination showed synergistic disruption under lower concentrations. applied this surgical specimens from three patients similar results. This facilitates test amounts fractionated patient's
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