New strategy for the analysis of phenotypic marker antigens in brain tumor-derived neurospheres in mice and humans.

摘要: Object Brain tumor stem cells (TSCs) hypothetically drive the malignant phenotype of glioblastoma multiforme (GBM), and evidence suggests that a better understanding these TSCs will have profound implications for treating gliomas. When grown in vitro, putative grow as solid sphere, making their subsequent characterization, particularly within center difficult. Therefore, purpose this study was to develop new method understand proteomic profile entire population sphere. Methods Tumor specimens from patients with confirmed GBM glioma models mice were mechanically enzymatically dissociated traditional cell medium generate neurospheres. The neurospheres then embedded freezing medium, cryosectioned, analyzed immunofluorescence. Results By sectioning thinly 5 μm, authors overcame many problems associated immunolabeling whole neurospheres, such...