Purification and Characterization of an Inducible s-Triazine Hydrolase from Rhodococcus corallinus NRRL B-15444R.

摘要: The widespread use and relative persistence of s-triazine compounds such as atrazine simazine have led to increasing concern about environmental contamination by these compounds. Few microbial isolates capable transforming substituted s-triazines been identified. Rhodococcus corallinus NRRL B-15444 has previously shown possess a hydrolase activity that is responsible for the dechlorination triazine deethylsimazine (6-chloro-N-ethyl-1,3,5-triazine-2,4-diamine) (CEAT) deethylatrazine (6-chloro-N-isopropyl-1,3,5-triazine-2,4-diamine) (CIAT). enzyme this was purified be composed four identical subunits 54,000 Da. Kinetic experiments revealed also deaminating structurally related melamine (2,4,6-triamino-1,3,5-triazine) (AAAT) CAAT (2-chloro-4,6-diamino-1,3,5-triazine), well pyrimidine 2,4,6-triaminopyrimidine (AAAP) 4-chloro-2,6-diaminopyrimidine (CAAP). herbicides inhibit hydrolytic activities but are not substrates. Induction demonstrate inducible rises approximately 20-fold during induction.